LABORATORY FOR CHROMATOGRAPHY
PROJECT BG05M2OP001-1.001-0008 | NATIONAL CENTER OF MECHATRONICS AND CLEAN TECHNOLOGIES
Manager: Prof. Margarita Popova
Contacts
e-mail: mpopova@orghm.bas.bg
phone: +359 2 9606 111
Chromatography is а method widely used for qualitative and quantitative analysis of organic compounds. Gas chromatography (GC) with TCD and/or FID is mainly used for analysis of volatile organic compounds and gas mixtures, while high performance liquid chromatography (HPLC) with a photodiode array detector is applied for characterization of compounds containing chromophore groups. Chromatographic analysis of organic compounds and mixtures obtained during biomass processing performed in liquid or gas phase is within the framework of the Center of Excellence. Analysis of new functional organic materials derived from target oriented synthesis with potential in advanced technologies and of volatile organic compounds (toluene, ethyl acetate, hexane, ethylbenzene, etc.) investigating their possible elimination with an environmental focus is planned as well.
ACTIVITIES
- Development of green technologies for the valorization of biomass and petroleum fractions in the presence of heterogeneous catalysts, such as metal/metal oxides modified mesoporous silicates, nano-sized metal oxides, hierarchical zeolites and others;
- Study of processes for elimination of volatile organic compounds in the presence of nano-sized metaloxide catalysts and micro/mesoporous materials supported by metaloxide particles;
- Design, synthesis, purification and characterization of pure compounds and mixtures with application in pharmacy and materials chemistry. These products can possess potential biological activity or desirable magnetic, optical, structural or catalytic properties. Based on current experience and after conducting in-depth literary inquiries the team has so far solved a number of complex tasks and problems;
- Analyses that follow the most up-to-date procedures used to control processes, assess and ensure quality. We combine and develop project-specific methods of analysis for this purpose. Our practical experience in development of analytical methods with liquid and gas chromatography while monitor complex reaction processes, including with chiral compounds, are the solid foundation to get reliable results and solutions in short time.
SERVICES
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Design and synthesis
- Synthesis of organometallic compounds and main groups and transition metals complexes, using also Schlenk techniques;
- Development of efficient synthetic strategies for multifunctional organic compounds – intermediates, key building blocks and target compounds with biological activity and/or desirable catalytic, magnetic, structural or optical properties;
- Solvent free and microwave syntheses of multifunctional heterocyclic compounds, including of chiral compounds; - Separation and purification of mixtures of synthetic products;
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Analysis of compounds and complex mixtures using liquid and gas chromatography;
- Quantitative and qualitative analysis of complex mixtures;
- Structure elucidation of novel organic compounds;
- Chiral liquid and gas chromatography analysis of enantiomeric excess of chiral substances, as well as configuration in the presence of a standard; - Analysis of gas mixtures containing volatile organic compounds, hydrogen, CO, CO2 and others;
- Analysis of organic compounds used as biofuels and biopolymers obtained in the presence of heterogeneous catalysts, such as modified with metal/metal oxides mesoporous silicates, nano-sized metal oxides, hierarchical zeolites and others.
EQUIPMENT
GAS CHROMATOGRAPH SYSTEM SHIMADZU NEXIS GC-2030
The gas chromatographs are designed for chromatographic separation of a wide range of volatile organic compounds and gas mixtures (H2, CO, CO2) using a thermal conductivity detector and with a possibility for quantitative analysis of individual components by flame ionization detection. Direct connection of the gas chromatographs to flow catalytic systems via six-port gas valves permits on-line analysis of the reaction products during catalytic processes, while the thermal conductivity detector allows the analysis of products containing CO, CO2, H2 gas mixtures and others. The system also includes a gas chromatograph, equipped with an autosampler, which considerably increases the number of samples to be analyzed thus improving the efficiency of the equipment.
HIGHLY EFFICIENT LIQUID CHROMATCHROMATOGRAPHIC SYSTEM Nexera-i LC-2040C 3D Plus
The SHIMADZU LC-2040C high performance liquid chromatograph is equipped with a photodiode array detector (PDA), which allows chromatographic separation of a wide range of complex mixtures of organic compounds as well as their qualitative and quantitative analysis by UV detection. The HPLC is also equipped with an automatic injector, which provides a significant increase in the number of samples analyzed and considerably increases the efficiency of the equipment. It is widely used for the analysis of novel organic compounds possessing chromophore groups obtained from biomass as well as of such derived from directed organic synthesis for application in cutting edge technologies.